An experiment testing the wavelength and intensity of light on the rate of photosynthesis

Abstract Photosynthesis in plants is affected by the intensity of the light the plant is exposed to. For this experiment, DCPIP was added to cuvettes with spinach chloroplasts, which were exposed to an incandescent light at different distances for different intervals of time. After each exposure, the cuvettes were placed in a spectrophotometer set at nm and the absorbance of DCPIP was measured. At 37 mm away from the light, the absorbance decreased at 0.

Thread, blade, stand with clamp etc. Set up an experiment using distilled water and Hydrilla plants in the beaker and a funnel fitted with jet-glass tube. Place the experimental set-up in sunlight for 30 min and record the volume of oxygen collected, if any, in the graduated tube.

If graduated tube is not available fit a nozzle at the tip of the funnel and count the no. Put mg of KHCO3 salt into the water of the beaker, stir and wait for 15 to 20 minutes and then record the volume of oxygen collected in the graduated tube.

In the same manner, at an interval of 30 minutes, add another mg of KHCO3 salt to the beaker and record the photosynthetic rate in terms of the volume of oxygen generated up to a considerable length of time and concentration of KHCO3 salt.

Graphically plot the data: Even at high CO2 concentration, initially there is saturation effect followed by gradual decrease of photosynthesis.

Context for Use

Initially the rise of photosynthetic rate due to CO2 is maintained for a considerable length of time at a particular light intensity and temperature. Then saturation of photosynthetic rate is obtained. Finally, at high CO2 concentration, photosynthetic rate decreases due to: Study of the Effect of Monochromatic Light on Photosynthesis: The quality, intensity and duration of exposure to light are some of the principal limiting factors of photosynthetic process in green plants.

An experiment testing the wavelength and intensity of light on the rate of photosynthesis

A very small fraction of light wavelength can be trapped by the green plants and utilized in photosynthesis. Thus the action spectrum varies widely with reference to the variation of monochromatic light. However, the photosynthetic efficiency decreases very dramatically with increasing wavelength beyond nm even though chlorophyll still absorbs light at these wavelengths.

This fact is the so-called red drop in photosynthesis. Beakers 1 lit capacityfunnel fitted with jets, graduated glass tubes, glass rods 2.

Investigating factors affecting the rate of photosynthesis | Nuffield Foundation

Illuminated chambers Blue, Green, Yellow, Red 5. Thread, blade, graph paper, pencil, stop-watch, etc. Prepare 4 photosynthetic experimental sets using Hydrilla plants in beakers in the usual manner.

An experiment testing the wavelength and intensity of light on the rate of photosynthesis

Add traces of KHCO3 salt in each set. Place the experimental sets in separate illuminated chambers Blue, Green, Yellow, Red for artificial exposure to light for an hour. Record the evolution of O2 as a result of photosynthesis separately in each experimental set and graphically plot the data against the respective light wavelengths for comparative analysis of the effect of monochromatic light.

For individual wavelength of light, the photosynthesis rate varies. The higher rate is seen in red light zone nm. The relative efficiency of light absorption and its subsequent utilization in photosynthesis depends on a number of factors: Effect of Temperature on Photosynthesis: Although the photochemical part of photosynthesis is independent of temperature, the biochemical part — which is controlled by enzyme activity — is strictly temperature-dependent.

However, there appears to be wide variance and adaptability among plants in their ability to tolerate temperature extremes. Materials and Equipments Required: Beakers 1 lit capacityfunnels fitted with jets, graduated test tubes, glass rods 2.#41 Effect of Light intensity on the rate of Photosynthesis.

Experiment. Place a pond weed Elodea upside in a test tube containing water. Place the tube in a beaker of fresh water at 25°C. This helps to maintain a constant temperature around the pond weed. A very small fraction of light wavelength can be trapped by the green plants and utilized in photosynthesis.

Further, the efficiency of photosynthesis (as measured by O 2 evolution) by monochromatic light (as a function of the wavelength of light) is termed ‘action spectrum’.

The Effect of Light Intensity and Temperature on the Rate of Photosynthesis - The Effect of Light Intensity and Temperature on the Rate of Photosynthesis Aim The aim of my experiment is to determine whether intensity of light and temperature would affect the rate of photosynthesis in .

Abstract: The purpose of the experiment was to determine the wavelength and light intensity effect on the reaction rate of photosynthesis.

Learning Goals

Photosynthesis is the conversion of light energy to chemical energy in the form of organic compounds. The light energy that is essential in this process is absorbed by photosynthetic pigments in the plant.

These pigments are used by plants to convert light energy into chemical energy, such as glucose. ("Investigating the Link Between Wavelength of Light and Rate of Photosynthesis.") For Elodea, the main pigment is chlorophyll. ("Investigating the Link Between Wavelength of Light and Rate of Photosynthesis.") For Elodea, the main pigment is chlorophyll.

Certain pigments are better at absorbing certain wavelengths of light, and for chlorophyll, blue and red lights are more easily absorbed while green is reflected off it.

QUESTIONS: 1. Propose a reason the bicarbonate solution, rather than water, is used during the photosynthesis reaction 2. Include a graph of your data from the light intensity experiment. Introduction: The rate of photosynthesis varies greatly with changes in wavelengths of light. Light's colour is determined by its wavelength of light, and thus it is possible to devise an experiment to determine which wavelengths of light are most productive for photosynthesis than others. Besides the light factor I also decided to put a watt lamp above each plant so I get a high light intensity to speed up the rate of photosynthesis. Aim: The purpose of this experiment is to find out under what wavelength (different colour of light) the elodea plants .
Light wavelength effect on the photosynthetic rate of elodea | Essay Example